Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Activity of immobilized papain dehydrated by n-propanol in low- water media

Theppakorn, T. and Kanasawud, P. and Halling, P.J. (2004) Activity of immobilized papain dehydrated by n-propanol in low- water media. Biotechnology Letters, 26 (2). pp. 133-136. ISSN 0141-5492

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degreesC. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.

Item type: Article
ID code: 313
Keywords: immobilized papain, low-water media, propanol-rinsed enzyme preparations, Organic-solvents, enzyme-activity, buffers, Chemistry
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Users 16 not found.
    Date Deposited: 10 Mar 2006
    Last modified: 12 Mar 2012 10:35
    URI: http://strathprints.strath.ac.uk/id/eprint/313

    Actions (login required)

    View Item