Picture of two heads

Open Access research that challenges the mind...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Effect of solid-state buffers on the catalytic activity of papain in low water media

Theppakorn, T. and Kanasawud, P. and Halling, P.J. (2003) Effect of solid-state buffers on the catalytic activity of papain in low water media. Enzyme and Microbial Technology, 32 (7). pp. 828-836. ISSN 0141-0229

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.