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Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

McGuinness, Colin D. and Macmillan, Alexander M. and Sagoo, Kulwinder and McLoskey, David and Birch, David J. S. (2006) Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers. Applied Physics Letters, 89 (6). 063901. ISSN 0003-6951

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Abstract

The authors describe the characteristics and application of a 265 nm AlGaN light-emitting diode (LED) operated at 1 MHz repetition rate, 1.2 ns pulse duration, 1.32 mu W average power, 2.3 mW peak power, and similar to 12 nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6-9.2, the authors find evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two fluorescent amino acids tryrosine and tryptophan than has previously been reported. (c) 2006 American Institute of Physics.

Item type: Article
ID code: 31132
Keywords: aluminium compounds, gallium compounds, light emitting diodes, biological techniques, Physics
Subjects: Science > Physics
Department: Faculty of Science > Physics
Related URLs:
    Depositing user: Pure Administrator
    Date Deposited: 23 Jun 2011 15:45
    Last modified: 17 Jul 2013 10:29
    URI: http://strathprints.strath.ac.uk/id/eprint/31132

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