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Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose

Rolinski, O.J. and Birch, D.J.S. and McCartney, L. and Pickup, J.C. (2001) Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 57 (11). pp. 2245-2254. ISSN 1386-1425

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Abstract

A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.

Item type: Article
ID code: 3060
Keywords: lifetime sensing, FRET, glucose, affinity sensor, diabetes mellitus, nanoscience, Solid state physics. Nanoscience, Instrumentation, Spectroscopy, Atomic and Molecular Physics, and Optics, Analytical Chemistry
Subjects: Science > Physics > Solid state physics. Nanoscience
Department: Faculty of Science > Physics
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 27 Mar 2007
    Last modified: 04 Sep 2014 10:30
    URI: http://strathprints.strath.ac.uk/id/eprint/3060

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