Rolinski, O.J. and Birch, D.J.S. and McCartney, L. and Pickup, J.C. (2001) Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 57 (11). pp. 2245-2254. ISSN 1386-1425Full text not available in this repository. (Request a copy from the Strathclyde author)
A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
|Keywords:||lifetime sensing, FRET, glucose, affinity sensor, diabetes mellitus, nanoscience, Solid state physics. Nanoscience, Instrumentation, Spectroscopy, Atomic and Molecular Physics, and Optics, Analytical Chemistry|
|Subjects:||Science > Physics > Solid state physics. Nanoscience|
|Department:||Faculty of Science > Physics|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||27 Mar 2007|
|Last modified:||29 Apr 2016 07:21|