Rolinski, O.J. and Birch, D.J.S. and McCartney, L. and Pickup, J.C. (2001) Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 57 (11). pp. 2245-2254. ISSN 1386-1425
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
| Item type: | Article |
|---|---|
| ID code: | 3060 |
| Keywords: | lifetime sensing, FRET, glucose, affinity sensor, diabetes mellitus, nanoscience, Solid state physics. Nanoscience |
| Subjects: | Science > Physics > Solid state physics. Nanoscience |
| Department: | Faculty of Science > Physics |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 27 Mar 2007 |
| Last modified: | 04 Oct 2012 15:20 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/3060 |
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