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Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex

Rolinski, Olaf J. and Birch, David J.S. and McCartney, Lydia J. and Pickup, John C. (2001) Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex. Physics in Medicine and Biology, 46 (2001). N221-N226. ISSN 0031-9155

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Abstract

A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalinA labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.