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Structural information on nanomolecular systems revealed by FRET

Rolinski, Olaf J. and Mathivanan, Chinnaraj and McNaught, Gillian and Birch, David J.S. (2004) Structural information on nanomolecular systems revealed by FRET. Biosensors and Bioelectronics, 20 (3). pp. 424-430. ISSN 0956-5663

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Abstract

Our newly developed fluorescence resonance energy transfer (FRET) based technique, fluorescence nanotomography (FN), is used to determine the morphology and dynamics of some soft materials and bio-molecules by attaching donor (D) fluorophores and acceptors (A) to the investigated structure and using fluorescence lifetime measurements to reveal the D-A distance distribution function ρDA(r).We report the effect of the limited sizes of the donor and acceptor, effect of porous polymer, and molecular structure and phase transition in phospholipid bilayers.