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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

Saxl, Tania and Khan, Faaizah and Ferla, Matteo and Birch, David and Pickup, John (2011) A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein. Analyst, 136 (5). pp. 968-972. ISSN 0003-2654

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Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP-Badan-beads. Either GBP-Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent K-d of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.

Item type: Article
ID code: 30189
Keywords: resonance-energy transfer, in-vivo, biosensor, micocapsules, assay, TIC - Bionanotechnology, Physical and theoretical chemistry, Biochemistry, Spectroscopy, Environmental Chemistry, Analytical Chemistry, Electrochemistry
Subjects: Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Physics
Technology and Innovation Centre > Bionanotechnology
Depositing user: Pure Administrator
Date Deposited: 30 Mar 2011 09:51
Last modified: 16 Oct 2015 17:44

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