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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

Saxl, Tania and Khan, Faaizah and Ferla, Matteo and Birch, David and Pickup, John (2011) A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein. Analyst, 136 (5). pp. 968-972. ISSN 0003-2654

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    Abstract

    Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP-Badan-beads. Either GBP-Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent K-d of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.

    Item type: Article
    ID code: 30189
    Keywords: resonance-energy transfer, in-vivo, biosensor, micocapsules, assay, TIC - Bionanotechnology, Physical and theoretical chemistry, Biochemistry, Spectroscopy, Environmental Chemistry, Analytical Chemistry, Electrochemistry
    Subjects: Science > Chemistry > Physical and theoretical chemistry
    Department: Faculty of Science > Physics
    Technology and Innovation Centre > Bionanotechnology
    Related URLs:
      Depositing user: Pure Administrator
      Date Deposited: 30 Mar 2011 10:51
      Last modified: 27 Mar 2014 11:14
      URI: http://strathprints.strath.ac.uk/id/eprint/30189

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