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Measurement and modelling of light scattering by phytoplankton cells at narrow forward angles

MacCallum, Iain and Cunningham, Alex and McKee, David (2004) Measurement and modelling of light scattering by phytoplankton cells at narrow forward angles. Journal of Optics A: Pure and Applied Optics, 6. pp. 698-702. ISSN 1464-4258

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Abstract

A procedure has been devised for measuring the angular dependence of light scattering from suspensions of phytoplankton cells at forward angles from 0.25◦ to 8◦. The cells were illuminated with a spatially-filtered laser beam and the angular distribution of scattered light measured by tracking a photodetector across the Fourier plane of a collecting lens using a stepper-motor driven stage. The procedure was calibrated by measuring scattering from latex bead suspensions with known size distributions. It was then used to examine the scattering from cultures of the unicellular algae Isochrysis galbana (4 μm × 5 μm), Dunaliella primolecta (6 μm × 7 μm) and Rhinomonas reticulata (5 μm × 11 μm). The results were compared with the predictions of Mie theory. Excellent agreement was obtained for spherical particles. A suitable choice of spherical-equivalent scattering parameters was required to enable reasonable agreement within the first diffraction lobe for ellipsoidal particles.

Item type: Article
ID code: 2912
Keywords: diffraction and scattering, Fourier optics, optical properties of seawater, photonics, Solid state physics. Nanoscience, Atomic and Molecular Physics, and Optics
Subjects: Science > Physics > Solid state physics. Nanoscience
Department: Unknown Department
Faculty of Science > Physics
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 15 Feb 2007
    Last modified: 04 Sep 2014 12:42
    URI: http://strathprints.strath.ac.uk/id/eprint/2912

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