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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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Measurement and modelling of light scattering by phytoplankton cells at narrow forward angles

MacCallum, Iain and Cunningham, Alex and McKee, David (2004) Measurement and modelling of light scattering by phytoplankton cells at narrow forward angles. Journal of Optics A: Pure and Applied Optics, 6. pp. 698-702. ISSN 1464-4258

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Abstract

A procedure has been devised for measuring the angular dependence of light scattering from suspensions of phytoplankton cells at forward angles from 0.25◦ to 8◦. The cells were illuminated with a spatially-filtered laser beam and the angular distribution of scattered light measured by tracking a photodetector across the Fourier plane of a collecting lens using a stepper-motor driven stage. The procedure was calibrated by measuring scattering from latex bead suspensions with known size distributions. It was then used to examine the scattering from cultures of the unicellular algae Isochrysis galbana (4 μm × 5 μm), Dunaliella primolecta (6 μm × 7 μm) and Rhinomonas reticulata (5 μm × 11 μm). The results were compared with the predictions of Mie theory. Excellent agreement was obtained for spherical particles. A suitable choice of spherical-equivalent scattering parameters was required to enable reasonable agreement within the first diffraction lobe for ellipsoidal particles.