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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Nanoparticle metrology in sol-gels using multiphoton excited fluorescence

Karolin, J. and Geddes, C.D. and Wynne, Klaas and Birch, D.J.S. (2001) Nanoparticle metrology in sol-gels using multiphoton excited fluorescence. Measurement Science and Technology, 13 (1). pp. 21-27. ISSN 0957-0233

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Abstract

We have developed a method of measuring the growth of nanoparticles during sol-gel glass formation based on labelling the particle with a fluorescent dye and determining the multiphoton excited decay of fluorescence anisotropy due to Brownian rotation. Multiphoton excitation is shown to give a higher dynamic range of measurement than one-photon excitation. We illustrate the sub-nanometre resolution and stability of our approach by detecting a 0.8-1.1 nm silica particle hydrodynamic mean radius increase in a tetramethylorthosilicate sol at pH 2.3 labelled with rhodamine 6G and observed over ≈4 weeks and also with a stable silica colloid of radius 6 nm, pH 8.9, labelled with a 6-methoxyquinoline-type dye.