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Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system

Sandison, Mairi E. and Zagnoni, Michele and Abu-Hantash, Mustafa and Morgan, Hywel (2007) Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system. Journal of Micromechanics and Microengineering, 17 (7). S189-S196. ISSN 0960-1317

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Abstract

The use of spark assisted chemical engraving (SACE) to produce glass apertures that are suitable for the formation of artificial bilayer lipid membranes is described. Prior to use, the glass apertures were rendered hydrophobic by a silanization process and were then incorporated into a simple microfluidic device. Successful bilayer lipid membrane (BLM) formation and the subsequent acquisition of single-channel recordings are demonstrated. Due to the simplicity and rapidity of the SACE process, these glass apertures could be easily integrated into an all-glass microfluidic system for BLM formation.