Picture of a sphere with binary code

Making Strathclyde research discoverable to the world...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. It exposes Strathclyde's world leading Open Access research to many of the world's leading resource discovery tools, and from there onto the screens of researchers around the world.

Explore Strathclyde Open Access research content

Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging

Bisby, R. H. and Botchway, S. W. and Crisostomo, A. G. and Karolin, J. and Parker, A. W. and Schroeder, L. (2010) Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging. Spectroscopy, 24 (1-2). pp. 137-142. ISSN 0712-4813

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the fluorescence spectrum and an increase in fluorescence lifetime. Quenching of propranolol fluorescence by iodide is used to demonstrate interaction with beta-cyclodextrin. At high concentrations, self-quenching of propranolol fluorescence was also observed with kappa(q), = 2.5 x 10(9) dm(3) mol(-1) s(-1). Two-photon excited (630 nm) fluorescence lifetime imaging of propranolol in cells showed propranolol to be widely distributed in the cell cytoplasm, with fluorescence lifetimes shorter than in solution. The results suggest that intracellular propranolol is mainly confined within the aqueous cytoplasm and rather than membrane associated.