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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

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Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging

Bisby, R. H. and Botchway, S. W. and Crisostomo, A. G. and Karolin, J. and Parker, A. W. and Schroeder, L. (2010) Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging. Spectroscopy, 24 (1-2). pp. 137-142. ISSN 0712-4813

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Abstract

Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the fluorescence spectrum and an increase in fluorescence lifetime. Quenching of propranolol fluorescence by iodide is used to demonstrate interaction with beta-cyclodextrin. At high concentrations, self-quenching of propranolol fluorescence was also observed with kappa(q), = 2.5 x 10(9) dm(3) mol(-1) s(-1). Two-photon excited (630 nm) fluorescence lifetime imaging of propranolol in cells showed propranolol to be widely distributed in the cell cytoplasm, with fluorescence lifetimes shorter than in solution. The results suggest that intracellular propranolol is mainly confined within the aqueous cytoplasm and rather than membrane associated.