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Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging

Bisby, R. H. and Botchway, S. W. and Crisostomo, A. G. and Karolin, J. and Parker, A. W. and Schroeder, L. (2010) Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging. Spectroscopy, 24 (1-2). pp. 137-142. ISSN 0712-4813

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Abstract

Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the fluorescence spectrum and an increase in fluorescence lifetime. Quenching of propranolol fluorescence by iodide is used to demonstrate interaction with beta-cyclodextrin. At high concentrations, self-quenching of propranolol fluorescence was also observed with kappa(q), = 2.5 x 10(9) dm(3) mol(-1) s(-1). Two-photon excited (630 nm) fluorescence lifetime imaging of propranolol in cells showed propranolol to be widely distributed in the cell cytoplasm, with fluorescence lifetimes shorter than in solution. The results suggest that intracellular propranolol is mainly confined within the aqueous cytoplasm and rather than membrane associated.

Item type: Article
ID code: 28998
Keywords: propranolol, fluorescence, lifetime, quenching, micelle, cyclodextrin, imaging, live cell, binding, water, Chemistry, Microbiology, Chemical technology, Spectroscopy
Subjects: Science > Chemistry
Science > Microbiology
Technology > Chemical technology
Department: Faculty of Science > Physics
Related URLs:
Depositing user: Pure Administrator
Date Deposited: 07 Mar 2011 23:22
Last modified: 27 Mar 2014 09:13
URI: http://strathprints.strath.ac.uk/id/eprint/28998

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