Picture of wind turbine against blue sky

Open Access research with a real impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

The Energy Systems Research Unit (ESRU) within Strathclyde's Department of Mechanical and Aerospace Engineering is producing Open Access research that can help society deploy and optimise renewable energy systems, such as wind turbine technology.

Explore wind turbine research in Strathprints

Explore all of Strathclyde's Open Access research content

Selective excitation of tryptophan fluorescence decay in proteins using a sub-nanosecond 295 nm light-emitting diode and time-correlated single-photon counting

McGuiness, C.D. and Sagoo, K. and McLoskey, D. and Birch, D.J.S. (2005) Selective excitation of tryptophan fluorescence decay in proteins using a sub-nanosecond 295 nm light-emitting diode and time-correlated single-photon counting. Applied Physics Letters, 86. p. 261911. ISSN 0003-6951

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

We demonstrate an AlGaN light-emitting diode (LED) giving pulses of ~600 ps full width half maximum, 0.35 W average power, 0.6 mW peak power, and ~12 nm bandwidth at 295 nm. This source is ideal for protein intrinsic tryptophan fluorescence decay research without the unwanted excitation of tyrosine and paves the way to lab-on-a-chip protein assays using fluorescence decay times. Fluorescence decay and anisotropy decay measurements of human serum albumin are reported and the usefulness of the 295 nm LED demonstrated in comparisons with a nanosecond flashlamp and LEDs with nominal wavelength emission of 280 nm.