Sinclair, J.A. and Henderson, C.J. and Martin, I.J. and Grant, M.H. and Tettey, J.N.A. (2007) Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. Journal of Pharmacy and Pharmacology, 59 (S1). A16. ISSN 0022-3573
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
| Item type: | Article |
|---|---|
| ID code: | 28462 |
| Keywords: | Bioengineering, Engineering (General). Civil engineering (General) |
| Subjects: | Technology > Engineering (General). Civil engineering (General) > Bioengineering Technology > Engineering (General). Civil engineering (General) |
| Department: | Faculty of Engineering > Bioengineering Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Related URLs: | |
| Depositing user: | Ms Ashley Urie |
| Date Deposited: | 23 Oct 2010 14:19 |
| Last modified: | 12 Mar 2012 11:21 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/28462 |
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