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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

Sinclair, J.A. and Henderson, C.J. and Martin, I.J. and Grant, M.H. and Tettey, J.N.A. (2007) Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. Journal of Pharmacy and Pharmacology, 59 (S1). A16. ISSN 0022-3573

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Abstract

Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.