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Efficient transesterification of sucrose catalysed by the metalloprotease thermolysin in dimethylsulfoxide

Pedersen, N.R. and Halling, P.J. and Pedersen, L.H. and Wimmer, R. and Matthiesen, R. and Veltman, O.R. (2002) Efficient transesterification of sucrose catalysed by the metalloprotease thermolysin in dimethylsulfoxide. FEBS Letters, 519 (1-3). pp. 181-184. ISSN 0014-5793

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Abstract

Thermolysin catalyses the formation of sucrose esters from sucrose and vinyl laurate in dimethylsulfoxide, with a specific activity of 53 nmol/min/mg and 2-O-lauroyl-sucrose as the main product. Such transesterification reactions are normally observed only when the mechanism involves an acyl enzyme intermediate, as with lipases or serine proteases, and not with metalloproteases like thermolysin. A possible reason is the affinity of the active site of thermolysin for sugar moieties, as for the potent inhibitor phosphoramidon. The reaction is not catalysed by other proteins under the same conditions, and is inhibited by removal of the active site zinc.

Item type: Article
ID code: 279
Keywords: transesterification, thermolysin, carbohydrate fatty acids ester, dimethylsulfoxide, reaction mechanism Enzymatic peptide-synthesis, regioselective esterification, lipase, acylation, protease, derivatives, alcohol, Chemistry
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
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    Depositing user: Users 16 not found.
    Date Deposited: 13 Mar 2006
    Last modified: 04 Oct 2012 15:25
    URI: http://strathprints.strath.ac.uk/id/eprint/279

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