Picture of person typing on laptop with programming code visible on the laptop screen

World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

Explore

Thermal degradation of polyethylene glycol 6000 and its effect on the assay of macroprolactin

Boughen, Louise and Liggat, John and Ellis, Graham (2010) Thermal degradation of polyethylene glycol 6000 and its effect on the assay of macroprolactin. Clinical Biochemistry, 43 (9). pp. 750-753. ISSN 0009-9120

[img] Microsoft Word (CB_2010_43_750.doc)
CB_2010_43_750.doc - Accepted Author Manuscript
License: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 logo

Download (173kB)

Abstract

Study of the effectiveness of partially degraded polyethylene glycol 6000 (PEG) as a precipitant for macroprolactin. PEG was heated to 63 °C in air for up to 20 days and its effectiveness assessed as a precipitant for sera containing normal prolactin or macroprolactin. Decomposition was studied chemically and with NMR spectroscopy. Thermal degradation was similar to what had occurred over several years of natural degradation. Initially PEG degraded 2-5 days caused excess precipitation of monomeric prolactin (false-positive macroprolactinemia). Samples degraded 18-20 days failed to precipitate macroprolactin, giving false negative results. Two 1H NMR peaks at 4-4.5 ppm were not detectable in undegraded PEG but were after 1 day. Their relative integral increased to 20 days. Aging of PEG can be accelerated by heating. The suitability of PEG for use in macroprolactin assays can be assessed by the absence of peaks at 4-4.5 ppm by 1H NMR.