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Stability of protein-coated microcrystals in organic solvents

Kreiner, M. and Fernandes, J.F.A. and O'Farrell, N. and Halling, P.J. and Parker, M.C. (2005) Stability of protein-coated microcrystals in organic solvents. Journal of Molecular Catalysis B: Enzymatic, 33 (3-6). pp. 65-72. ISSN 1381-1177

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Abstract

Previously we reported a new high activity biocatalyst for use in organic media, termed protein-coated microcrystals (PCMC) [M. Kreiner, B.D. Moore, M.C. Parker, Chem. Commun. 12 (2001) 1906]. These novel biocomposites consist of water-soluble micron-sized crystalline particles coated with the given biocatalyst(s). Here we have looked at the stability of PCMC and their catalytic behaviour as a function of temperature in different organic media. PCMC show very good long-term stability at room temperature, when stored as suspensions in 1-propanol/I wt.% H2O. Candida antarctica lipase B and subtilisin Carlsberg (SC) in PCMC form retained nearly 90% of their initial activity after 1 year at room temperature (RT). The effects of temperature on the catalytic activity of SC-PCMC are solvent-dependant. In 1-propanol/1 wt.% H2O, the initial rate increased when the temperature was elevated from 25 to 60 degrees C, whereas in acetonitrile/1 wt.% H2O, SC-PCMC lost activity. The operational stability of PCMC is also solvent-dependant. In 1-propanol/I wt.% H2O, SC-PCMC lost only 16% of the initial activity after five batch cycles. Rather poor stability was found for SC-PCMC in THF/1% (v/v) H2O and acetonitrile/1% (v/v) H2O, with a rapid loss of activity within 4h in a continuous flow reactor. However, during the next 4 days only a slow further deactivation was observed.

Item type: Article
ID code: 276
Keywords: protein-coated microcrystals(PCMC), crystalline particles, organic media, antarctica lipase B, Stability, Organic solvent, Subtilisin, Lipase, Chemistry
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Users 16 not found.
    Date Deposited: 14 Mar 2006
    Last modified: 12 Mar 2012 10:35
    URI: http://strathprints.strath.ac.uk/id/eprint/276

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