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The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

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Stability of protein-coated microcrystals in organic solvents

Kreiner, M. and Fernandes, J.F.A. and O'Farrell, N. and Halling, P.J. and Parker, M.C. (2005) Stability of protein-coated microcrystals in organic solvents. Journal of Molecular Catalysis B: Enzymatic, 33 (3-6). pp. 65-72. ISSN 1381-1177

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Abstract

Previously we reported a new high activity biocatalyst for use in organic media, termed protein-coated microcrystals (PCMC) [M. Kreiner, B.D. Moore, M.C. Parker, Chem. Commun. 12 (2001) 1906]. These novel biocomposites consist of water-soluble micron-sized crystalline particles coated with the given biocatalyst(s). Here we have looked at the stability of PCMC and their catalytic behaviour as a function of temperature in different organic media. PCMC show very good long-term stability at room temperature, when stored as suspensions in 1-propanol/I wt.% H2O. Candida antarctica lipase B and subtilisin Carlsberg (SC) in PCMC form retained nearly 90% of their initial activity after 1 year at room temperature (RT). The effects of temperature on the catalytic activity of SC-PCMC are solvent-dependant. In 1-propanol/1 wt.% H2O, the initial rate increased when the temperature was elevated from 25 to 60 degrees C, whereas in acetonitrile/1 wt.% H2O, SC-PCMC lost activity. The operational stability of PCMC is also solvent-dependant. In 1-propanol/I wt.% H2O, SC-PCMC lost only 16% of the initial activity after five batch cycles. Rather poor stability was found for SC-PCMC in THF/1% (v/v) H2O and acetonitrile/1% (v/v) H2O, with a rapid loss of activity within 4h in a continuous flow reactor. However, during the next 4 days only a slow further deactivation was observed.