Picture of smart phone in human hand

World leading smartphone and mobile technology research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by Strathclyde researchers from the Department of Computer & Information Sciences involved in researching exciting new applications for mobile and smartphone technology. But the transformative application of mobile technologies is also the focus of research within disciplines as diverse as Electronic & Electrical Engineering, Marketing, Human Resource Management and Biomedical Enginering, among others.

Explore Strathclyde's Open Access research on smartphone technology now...

Effect of water activity on the rate profile of subtilisin Carlsberg in toluene in the presence of an organo-soluble acid- base buffer

Harper, N. and Dolman, M. and Moore, B.D. and Halling, P.J. (2001) Effect of water activity on the rate profile of subtilisin Carlsberg in toluene in the presence of an organo-soluble acid- base buffer. Enzyme and Microbial Technology, 29 (6-7). pp. 413-416. ISSN 0141-0229

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The ionisation state of the enzyme and the water activity (alpha (w)) of the system are two important factors which affect catalytic activity in low-water organic solvents. Here we investigate the interaction between effects of alpha (w) and the ionisation state of subtilisin Carlsberg, controlled by an organo-soluble dendrimer buffer. It was shown that alpha (w) does not have a major effect on the relationship between rate of transesterification in toluene and buffer ratio, [sodium salt]/[acid]. The weak dependence on alpha (w) (between 0.54 and 0.85) probably reflects compensating effects on ionisation equilibria for the buffer and the carboxyl groups on the enzyme. (C) 2001 Elsevier Science Inc. All rights reserved.