Fontes, N. and Almeida, M.C. and Garcia, S. and Peres, C. and Partridge, J. and Halling, P.J. and Barreiros, S. (2001) Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. Biotechnology Progress, 17 (2). pp. 355-358. ISSN 8756-7938Full text not available in this repository. (Request a copy from the Strathclyde author)
We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
|Keywords:||Organic-solvents, water activity, crystals, acetonitrile, hydration, buffers, ph, Chemistry, Biotechnology|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Users 16 not found.|
|Date Deposited:||14 Mar 2006|
|Last modified:||29 Apr 2016 07:22|