Fontes, N. and Almeida, M.C. and Garcia, S. and Peres, C. and Partridge, J. and Halling, P.J. and Barreiros, S. (2001) Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. Biotechnology Progress, 17 (2). pp. 355-358. ISSN 8756-7938
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.
| Item type: | Article |
|---|---|
| ID code: | 263 |
| Keywords: | Organic-solvents, water activity, crystals, acetonitrile, hydration, buffers, ph, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry |
| Related URLs: | |
| Depositing user: | Users 16 not found. |
| Date Deposited: | 14 Mar 2006 |
| Last modified: | 12 Mar 2012 10:35 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/263 |
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