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Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg

Fontes, N. and Almeida, M.C. and Garcia, S. and Peres, C. and Partridge, J. and Halling, P.J. and Barreiros, S. (2001) Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. Biotechnology Progress, 17 (2). pp. 355-358. ISSN 8756-7938

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Abstract

We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.

Item type: Article
ID code: 263
Keywords: Organic-solvents, water activity, crystals, acetonitrile, hydration, buffers, ph, Chemistry, Biotechnology
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
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Depositing user: Users 16 not found.
Date Deposited: 14 Mar 2006
Last modified: 04 Sep 2014 10:34
URI: http://strathprints.strath.ac.uk/id/eprint/263

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