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Essential roles of three enhancer sites in sigma-54-dependent transcription by the nitric oxide sensing regulatory protein NorR

Tucker, Nick and Ghosh, T. and Bush, M. and Zhang, X. and Dixon, Ray (2010) Essential roles of three enhancer sites in sigma-54-dependent transcription by the nitric oxide sensing regulatory protein NorR. Nucleic Acids Research, 38 (4). pp. 1182-1194. ISSN 0305-1048

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Abstract

The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. Unique to the norVW promoter in Escherichia coli is the presence of three enhancer sites associated with a binding site for 54-RNA polymerase. Here we show that all three sites are required for NorR-dependent catalysis of open complex formation by 54-RNAP holoenzyme (E54). We demonstrate that this is essentially due to the need for all three enhancers for maximal ATPase activity of NorR, energy from which is used to remodel the closed E54 complex and allow melting of the promoter DNA. We also find that site-specific DNA binding per se promotes oligomerisation but the DNA flanking the three sites is needed to further stabilise the functional higher order oligomer of NorR at the enhancers.

Item type: Article
ID code: 26105
Keywords: bacterial activator protein, gene transcription, Escherichia coli, DNA binding, nitric oxide, Genetics, Microbiology
Subjects: Science > Natural history > Genetics
Science > Microbiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Engineering > Electronic and Electrical Engineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 08 Sep 2010 11:59
    Last modified: 31 Oct 2012 16:59
    URI: http://strathprints.strath.ac.uk/id/eprint/26105

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