Flores, M.V. and Naraghi, K. and Engasser, J.M. and Halling, P.J. (2002) Influence of glucose solubility and dissolution rate on the kinetics of lipase catalyzed synthesis of glucose laurate in 2- methyl 2-butanol. Biotechnology and Bioengineering, 78 (7). pp. 814-820. ISSN 0006-3592Full text not available in this repository. (Request a copy from the Strathclyde author)
The lipase catalyzed acylation of glucose by dodecanoic acid in 2-methyl 2-butanol was studied. The initial reaction rate was strongly dependent on the dissolved glucose concentration in the medium. Several methods were shown to increase dissolved glucose concentrations and initial reaction rates, namely, the use of solid beta-glucose, amorphous solid glucose, and supersaturated glucose solution. Supersaturated glucose solutions in 2-methyl 2-butanol showed a high stability even in the presence of solid crystalline glucose. During the reaction, the dissolved glucose concentration falls as the reaction proceeds, before recovering later as more of the excess solid dissolves. However, the ester synthesis rate continues to fall even after glucose concentration reaches its minimum, so glucose dissolution rate limitation is not responsible for the synthesis rate decline. Experiments with added molecular sieves show that the main reason is the accumulation of product water. In the presence of molecular sieves, 70% of glucose was converted to ester, independent of the initial soluble glucose in the medium.
|Keywords:||sugar esters, nonaqueous media, lipase, glucose Fatty-acid esters, supersaturated substrate solutions, enzymatic-synthesis, organic-solvents, sugars, acylation, fructose, media, transformations, esterification, Chemistry, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry
|Depositing user:||Users 16 not found.|
|Date Deposited:||14 Mar 2006|
|Last modified:||28 Jul 2016 00:01|