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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

Raddatz, M.S.L. and Dolf, A. and Endl, E. and Knolle, P. and Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47 (28). pp. 5190-5193. ISSN 1433-7851

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Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.