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Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

Raddatz, M.S.L. and Dolf, A. and Endl, E. and Knolle, P. and Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47 (28). pp. 5190-5193. ISSN 1433-7851

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Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.

Item type: Article
ID code: 26096
Keywords: aptamers, c ell recognition, flow cytometry, fluorescence, in vitro selection, Other systems of medicine, Chemistry(all), Catalysis
Subjects: Medicine > Other systems of medicine
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 20 Aug 2010 16:45
    Last modified: 19 Aug 2014 13:53
    URI: http://strathprints.strath.ac.uk/id/eprint/26096

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