Raddatz, M.S.L. and Dolf, A. and Endl, E. and Knolle, P. and Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47 (28). pp. 5190-5193. ISSN 1433-7851
Full text not available in this repository. (Request a copy from the Strathclyde author)Official URL: http://dx.doi.org/10.1002/anie.200800216
Abstract
In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.
| Item type: | Article |
|---|---|
| ID code: | 26096 |
| Keywords: | aptamers, c ell recognition, flow cytometry, fluorescence, in vitro selection, Other systems of medicine |
| Subjects: | Medicine > Other systems of medicine |
| Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 20 Aug 2010 16:45 |
| Last modified: | 12 Mar 2012 11:16 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/26096 |
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