Singh, M.D. and Kreiner, M. and McKimmie, C.S. and Holt, S. and van der Walle, C.F. and Graham, G.J. (2009) Dimeric integrin a5ß1 ligands confer morphological and differentiation responses to murine embryonic stem cells. Biochemical and Biophysical Research Communications, 390 (3). pp. 716-721. ISSN 1090-2104Full text not available in this repository. (Request a copy from the Strathclyde author)
We present the first report utilizing, and showing the functional relevance of, self-assembling polyvalent ligands specific for integrin α5β1 in murine embryonic stem (mES) cell adhesion. Di, tri and tetrameric 9th-10th type III fibronectin domains (FIII9′10) were used to generate clustered integrin α5β1 ligand surfaces for mES cell culture. Compared to gelatin, FIII9′10 (monomer), FIII9′10-trimer and -tetramer, the FIII9′10-dimer supported the highest number of mES cell colonies. No evidence of domain unfolding upon surface adsorption was found. Colonies appeared disperse with a spread cell morphology unless subdued back to a tight morphology with increasing concentrations of leukemia inhibitory factor (LIF). In the presence of LIF, mES cells adherent to the FIII9′10-dimer showed transient upregulation of Oct-4, the mesodermal transcription factor, Brachyury, and the ectodermal marker, Nestin. However, dual upregulation of Nanog maintained the mES cells in a pluripotent state, confirmed by alkaline phosphatase staining. Therefore, the behavior of mES cells adherent to dimeric integrin α5β1 ligands is a largely morphological phenomenon conferring pro-differentiation signals towards mesodermal and ectodermal lineages. This work will be of interest to cell and tissue engineering groups aiming to control ES cell behavior through integrin ligand presentation and synthetic substrates.
|Keywords:||embryonic stem cells, cell adhesion, fibronectin, integrin, self-assembly, neutron reflectivity, Chemistry, Biochemistry, Cell Biology, Molecular Biology, Biophysics|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||01 Oct 2010 09:15|
|Last modified:||27 Apr 2016 15:46|