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Confocal Microscopy using an InGaN violet laser diode at 406nm

Girkin, J.M. and Ferguson, A.I. and Wokosin, D.L. and Gurney, A.M. (2000) Confocal Microscopy using an InGaN violet laser diode at 406nm. Optics Express, 7 (10). pp. 336-341. ISSN 1094-4087

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    Abstract

    We report on the application of a novel all-solid-state violet laser diode source to confocal microscopy. The source has the potential to replace argon ion lasers in a range of fluorescence based imaging systems. Improvements in system performance and image quality through the use of anamorphic prisms to modify the beam profile have been characterised. These modifications have permitted high quality, optically sectioned images to be obtained from laser diodes operating around 406nm. Living mammalian cells stained with a range of biologically significant fluorophores have been imaged. In addition, it has been shown that at this wavelength it is possible to image dyes that normally require excitation with UV argon laser lines.

    Item type: Article
    ID code: 26
    Notes: © Optical Society of America
    Keywords: Optics. Light, Atomic and Molecular Physics, and Optics
    Subjects: Science > Physics > Optics. Light
    Department: Faculty of Science > Institute of Photonics
    Faculty of Science > Physics
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and Pharmacology
    Related URLs:
      Depositing user: Mr N Joint
      Date Deposited: 02 Nov 2005
      Last modified: 04 Sep 2014 13:04
      URI: http://strathprints.strath.ac.uk/id/eprint/26

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