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Early endosomes and endosomal coatomer are required for autophagy

Razi, M. and Chan, Edmond Y. and Tooze, Sharon A. (2009) Early endosomes and endosomal coatomer are required for autophagy. Journal of Cell Biology, 185 (2). pp. 305-321. ISSN 0021-9525

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Abstract

Autophagy, an intracellular degradative pathway, maintains cell homeostasis under normal and stress conditions. Nascent double-membrane autophagosomes sequester and enclose cytosolic components and organelles, and subsequently fuse with the endosomal pathway allowing content degradation. Autophagy requires fusion of autophagosomes with late endosomes, but it is not known if fusion with early endosomes is essential. We show that fusion of AVs with functional early endosomes is required for autophagy. Inhibition of early endosome function by loss of COPI subunits (β′, β, or α) results in accumulation of autophagosomes, but not an increased autophagic flux. COPI is required for ER-Golgi transport and early endosome maturation. Although loss of COPI results in the fragmentation of the Golgi, this does not induce the formation of autophagosomes. Loss of COPI causes defects in early endosome function, as both transferrin recycling and EGF internalization and degradation are impaired, and this loss of function causes an inhibition of autophagy, an accumulation of p62/SQSTM-1, and ubiquitinated proteins in autophagosomes.

Item type: Article
ID code: 25986
Keywords: autophagy, cell homeostasis, Nascent double-membrane autophagosomes, autophagosomes, endosomes, Biology, Cell Biology
Subjects: Science > Natural history > Biology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Strathprints Administrator
Date Deposited: 07 Sep 2010 15:40
Last modified: 15 Apr 2015 11:00
URI: http://strathprints.strath.ac.uk/id/eprint/25986

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