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Translational modifications to improve vaccine efficacy in an oral influenza vaccine

Bennett, E. and Mullen, A.B. and Ferro, V.A. (2009) Translational modifications to improve vaccine efficacy in an oral influenza vaccine. Methods, 49 (4). pp. 322-327. ISSN 1046-2023

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Abstract

Oral vaccination using protein antigens is complicated by the degradative effects of the inhospitable conditions in the gastrointestinal tract, such as low pH and digestive enzymes, nescessitating protection and effective delivery of the antigen. The bilosome is a lipid-based, vesicle delivery system incorporating bile salts, which is believed to protect the antigen from degradation, and has been shown to induce significant antibody responses when delivered orally with various vaccines. In translational research, from laboratory bench to industrial scale-up, it is necessary to optimise the manufacturing process in order to improve efficiency and simplify production, giving a more economical end-product. In this study we tested two simplified production methods (3-step and 1-step) along with two different storage methods (lyophilised and non-lyophilised), as well as looking at the effect of buffer pH. The formulations were assessed in a murine system for immunogenicity, alongside characterisation in terms of size and antigen entrapment, with the stability of these aspects assessed with respect to time. Both lyophilised and non-lyophilised 3-step formulations induced significant IgG1, IgG2a and IgA titres, with the lyophilised version showing stable size and antigen entrapment up to 9 months.

Item type: Article
ID code: 25949
Keywords: oral vaccination, influenza, mucosal, pharmacology, Therapeutics. Pharmacology, Biochemistry, Genetics and Molecular Biology(all), Molecular Biology
Subjects: Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 20 Aug 2010 14:24
    Last modified: 05 Sep 2014 05:13
    URI: http://strathprints.strath.ac.uk/id/eprint/25949

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