Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Advances in imaging of new targets for pharmacological intervention in stroke : real time tracking of T cells in the ischaemic brain

Ortolano, F. and Maffia, Pasquale and Dever, G. and Rodolico, G. and Millington, O.R. and De Simoni, M.G. and Brewer, J.M. and Bushell, T. and Garside, P. and Carswell, H.V.O. (2010) Advances in imaging of new targets for pharmacological intervention in stroke : real time tracking of T cells in the ischaemic brain. British Journal of Pharmacology, 159 (4). pp. 808-811. ISSN 0007-1188

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

T-cells may play a role in the evolution of ischaemic damage and repair, but the ability to image these cells in the living brain after a stroke has been limited. We aim to extend the technique of real-time in situ brain imaging of T-cells, previously shown in models of immunological diseases, to models of experimental stroke. Male C57BL6 mice (6-8 weeks) (n= 3) received a total of 2-5 x 10(6) carboxyfluorescein diacetate succinimidyl ester (CFSE)-labelled lymphocytes from donor C57BL6 mice via i.v. injection by adoptive transfer. Twenty-four hours later, recipient mice underwent permanent left distal middle cerebral artery occlusion (MCAO) by electrocoagulation or by sham surgery under isoflurane anaesthesia. Female hCD2-green fluorescent protein (GFP) transgenic mice that exhibit GFP-labelled T-cells underwent MCAO. At 24 or 48 h post-MCAO, a sagittal brain slice (1500 microm thick) containing cortical branches of the occluded middle cerebral artery (MCA) was dissected and used for multiphoton laser scanning microscopy (MPLSM). Our results provide direct observations for the first time of dynamic T-cell behaviour in living brain tissue in real time and herein proved the feasibility of MPLSM for ex vivo live imaging of immune response after experimental stroke. It is hoped that these advances in the imaging of immune cells will provide information that can be harnessed to a therapeutic advantage.

Item type: Article
ID code: 25896
Notes: Strathprints' policy is to record up to 8 authors per publication, plus any additional authors based at the University of Strathclyde. More authors may be listed on the official publication than appear in the Strathprints' record.
Keywords: inflammation, leukocytes, brain imaging, middle cerebral artery occlusion, stroke, multiphoton microscopy, Optics. Light, Therapeutics. Pharmacology
Subjects: Science > Physics > Optics. Light
Medicine > Therapeutics. Pharmacology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 20 Sep 2010 16:37
    Last modified: 06 Dec 2013 23:50
    URI: http://strathprints.strath.ac.uk/id/eprint/25896

    Actions (login required)

    View Item