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Evaluation of nucleosome forming potentials (NFPs) of forensically important STRs

Thanakiatkrai, Phuvadol and Welch, Lindsey (2011) Evaluation of nucleosome forming potentials (NFPs) of forensically important STRs. Forensic Science International: Genetics, 5 (4). pp. 285-290. ISSN 1872-4973

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    Abstract

    Degraded forensic samples have proved difficult to analyze and interpret. New analysis techniques are constantly being discovered and improved but researchers have overlooked the structural properties that could prevent or slow the process of degradation. In theory, DNA that are bound to histones as nucleosomes are less prone to degradation, because nucleosomes prevent DNA from being exposed to degradative enzymes. In this study we determined the probability of 60 forensic DNA markers to be bound to histones based on their base sequence composition. Two web-based tools - NXSensor and nuScore - were used to analyze four hundred base pairs surrounding each DNA marker for properties that inhibit or promote the binding of DNA to histones. Our results showed that the majority of markers analyzed were likely to be bound as nucleosomes. Selection of the markers that are more protected to form a multiplex could increase the chance of obtaining a better balanced, easier to interpret DNA profile from degraded samples

    Item type: Article
    ID code: 25825
    Keywords: nucleosome, nucleosome forming potential, nucleosome positioning signal, forensic, STR, evaluation, NFPs, forensically important, STRs, Chemistry, Genetics, Biology, Genetics, Pathology and Forensic Medicine
    Subjects: Science > Chemistry
    Science > Natural history > Genetics
    Science > Natural history > Biology
    Department: Faculty of Science > Pure and Applied Chemistry
    Related URLs:
    Depositing user: Ms Lorraine Stewart
    Date Deposited: 09 Jul 2010 14:41
    Last modified: 27 Mar 2014 11:19
    URI: http://strathprints.strath.ac.uk/id/eprint/25825

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