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World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery

Erbeldinger, M. and Halling, P.J. and Ni, X.W. (2001) Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery. AIChE Journal, 47 (2). pp. 500-508. ISSN 0001-1541

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Abstract

The feasibility of scale-up for applications of enzymatic solid-to-solid synthesis of dipeptides was investigated by using Thermoase catalyzed synthesis of Z-Asp-Phe-OMe as a model reaction. The reaction system involves typically only 10% (w/w) enzyme suspension added to dry solid substrates. Enzymatic solid-to-solid biocatalysis combines the main advantages of aqueous media, high catalytic activity, low water media, and high yields. A flow sheet for the whole process is described, as well as the use of a jacketed reactor with an anchor stirrer for various steps of the process, including the neutralization reaction to adjust the acid-base condition of the reaction mixture. A solution for enzyme recycling, an important issue for scale-up applications, is also suggested.