Erbeldinger, M. and Halling, P.J. and Ni, X.W. (2001) Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery. AIChE Journal, 47 (2). pp. 500-508. ISSN 0001-1541Full text not available in this repository. (Request a copy from the Strathclyde author)
The feasibility of scale-up for applications of enzymatic solid-to-solid synthesis of dipeptides was investigated by using Thermoase catalyzed synthesis of Z-Asp-Phe-OMe as a model reaction. The reaction system involves typically only 10% (w/w) enzyme suspension added to dry solid substrates. Enzymatic solid-to-solid biocatalysis combines the main advantages of aqueous media, high catalytic activity, low water media, and high yields. A flow sheet for the whole process is described, as well as the use of a jacketed reactor with an anchor stirrer for various steps of the process, including the neutralization reaction to adjust the acid-base condition of the reaction mixture. A solution for enzyme recycling, an important issue for scale-up applications, is also suggested.
|Keywords:||Aspartame precursor, immobilized thermolysin, extractive reaction, organic-solvents, methyl-ester, conversion, water, Chemistry, Chemical Engineering(all), Biotechnology, Environmental Engineering|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry
|Depositing user:||Users 16 not found.|
|Date Deposited:||14 Mar 2006|
|Last modified:||29 Apr 2016 07:22|