Picture of athlete cycling

Open Access research with a real impact on health...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the Physical Activity for Health Group based within the School of Psychological Sciences & Health. Research here seeks to better understand how and why physical activity improves health, gain a better understanding of the amount, intensity, and type of physical activity needed for health benefits, and evaluate the effect of interventions to promote physical activity.

Explore open research content by Physical Activity for Health...

Aging and Ca2+ signaling in murine mesenteric arterial myocytes

Angermann, J.E. and Mcallister, C. and del Corsso, C. and Gurney, A.M. and Wilson, S.M. (2004) Aging and Ca2+ signaling in murine mesenteric arterial myocytes. FASEB Journal, 18 (4-5). A703-A703. ISSN 0892-6638

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

Vascular reactivity may be reduced in old individuals and the (Ca2+)i is important to smooth muscle contractility. Experiments were therefore performed to determine if Ca2+ signaling is altered in mesenteric arterial smooth muscle cells (MASMCs) from 5-6 (young (Y)) and 29-30 month (old (O)) C57bl/6 mice. (Ca2+)i was measured using fura-2 imaging of individual MASMCs. The basal (Ca2+)i of MASMCs from Y (36+/-6 nM n=48) and O (51+/-4 nM n=101) were not significantly different. Total cytosolic Ca2+ (CaT) released by 30s 10 mM caffeine (CAF) or 10 ?M phenylephrine (PE) exposures were estimated by integrating the Ca2+ transient. CaT was proportional to peak (Ca2+)i in both Y and O, but the slope of CaT to peak (Ca2+)i was 2-fold greater in Y for both CAF and PE. The (Ca2+)i increase above basal following SR Ca2+ depletion in Y 66+/-8 nM n=19 was greater than in O 18+/-3 nM n=34. Following store depletion the (Ca2+)i clearance rate (Mclear) with extracellular Ca2+ removal in Y 0.0073+/-0.0009 s-1 n=19 was slower than in O 0.0161+/-0.0008 s-1 n=34. The (Ca2+)i cleared from the cytosol (Mclear*CaT) was proportional to the peak (Ca2+)i and was the same between Y and O for CAF, while that due to PE was greater in O. Aging modifies agonist-induced cytosolic Ca2+ increases and our data imply that altered cytosolic Ca2+ clearance mechanisms are an important component to the Ca2+ signaling impairments. NIH P20 RR15581 from NCRR, AG20400, HL10476, AI55462.