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Development and validation of an improved hplc method for the control of potentially counterfeit isometamidium products

Schad, G.J. and Allanson, A. and Mackay, S.P. and Cannavan, A. and Tettey, J.N.A. (2008) Development and validation of an improved hplc method for the control of potentially counterfeit isometamidium products. Journal of Pharmaceutical and Biomedical Analysis, 46 (1). pp. 45-51. ISSN 0731-7085

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Abstract

Isometamidium, a mixture of related substances of which 8-(3-m-amidinophenyl-2-triazeno)-3-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4180A) is the principal active component, is the only chemical agent available for prophylaxis of veterinary trypanosomiasis. A method for the simultaneous quantitation of the major constituents M&B4180A, 3-(3-m-amidinophenyl-2-triazeno)-8-amino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B38897), 7-(m-amidinophenyldiazo)-3,8-diamino-5-ethyl-6-phenylphenanthridinium chloride hydrochloride (M&B4250) and 3,8-di(3-m-amidinophenyltriazeno)-5-ethyl-6-phenylphenanthridinium chloride dihydrochloride (M&B4596) is described. The related substances are resolved on a Gemini C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase composed of a mixture of acetonitrile and 50 mM ammonium formate buffer pH 2.8 (25:75 v/v) at a flow rate of 1 ml/min with UV detection at 320 nm. The method is compatible with electrospray ionisation mass spectrometry and provides a tool for the control of substandard and counterfeit commercial preparations of isometamidium.

Item type: Article
ID code: 19815
Keywords: isometamidium, HPLC, LC-MS, counterfeit medicines, related substances, Pharmacy and materia medica, Drug Discovery, Pharmaceutical Science, Spectroscopy, Analytical Chemistry, Clinical Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Unknown Department
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 27 May 2010 11:24
    Last modified: 05 Sep 2014 02:06
    URI: http://strathprints.strath.ac.uk/id/eprint/19815

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