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Optical trapping and manipulation of live T cells with a low numerical aperture lens

Harris, J. and McConnell, G. (2008) Optical trapping and manipulation of live T cells with a low numerical aperture lens. Optics Express, 16 (18). pp. 14036-14043. ISSN 1094-4087

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Abstract

An optical manipulation system that employs both optical andtemperature gradients to simultaneously enable trapping, manipulating and imaging of live cells with a low magnification, low numerical aperture objective lens (10x/0.4 N.A.) is reported. This approach negatesthe requirement for a high N.A. lens used in traditional optical trapping.Our system comprised a dual scanning system and two independent lasers which provided the ability to move the trapping spot independently of the confocal imaging process in close to real-time and without pre-programming. To demonstrate the efficacy of this innovativemethod, trapping and manipulation of live T cells was simultaneously performed over a field of view exceeding 1 mm2 for extended periods without compromising cell viability.

Item type: Article
ID code: 19508
Notes: http://suprimo.lib.strath.ac.uk/primo_library/libweb/action/search.do?scp.scps=scope%3A(SU)&srt=rank&tab=local&mode=Basic&dum=true&fn=search&frbg=&dstmp=1274283512723&dscnt=0&ct=search&vid=SUVU01&indx=1&vl(freeText0)=Optics%20Express&vl(54032236UI0)=lsr02&vl(96071691UI1)=all_items
Keywords: optical trapping, manipulation, live T cells, low numerical aperture lens, Ophthalmology, Optics. Light, Pharmacy and materia medica, Atomic and Molecular Physics, and Optics
Subjects: Medicine > Ophthalmology
Science > Physics > Optics. Light
Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Strathprints Administrator
Date Deposited: 19 May 2010 16:49
Last modified: 28 Mar 2014 05:17
URI: http://strathprints.strath.ac.uk/id/eprint/19508

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