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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Optical trapping and manipulation of live T cells with a low numerical aperture lens

Harris, J. and McConnell, G. (2008) Optical trapping and manipulation of live T cells with a low numerical aperture lens. Optics Express, 16 (18). pp. 14036-14043. ISSN 1094-4087

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Abstract

An optical manipulation system that employs both optical andtemperature gradients to simultaneously enable trapping, manipulating and imaging of live cells with a low magnification, low numerical aperture objective lens (10x/0.4 N.A.) is reported. This approach negatesthe requirement for a high N.A. lens used in traditional optical trapping.Our system comprised a dual scanning system and two independent lasers which provided the ability to move the trapping spot independently of the confocal imaging process in close to real-time and without pre-programming. To demonstrate the efficacy of this innovativemethod, trapping and manipulation of live T cells was simultaneously performed over a field of view exceeding 1 mm2 for extended periods without compromising cell viability.