Picture of two heads

Open Access research that challenges the mind...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Optical trapping and manipulation of live T cells with a low numerical aperture lens

Harris, J. and McConnell, G. (2008) Optical trapping and manipulation of live T cells with a low numerical aperture lens. Optics Express, 16 (18). pp. 14036-14043. ISSN 1094-4087

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

An optical manipulation system that employs both optical andtemperature gradients to simultaneously enable trapping, manipulating and imaging of live cells with a low magnification, low numerical aperture objective lens (10x/0.4 N.A.) is reported. This approach negatesthe requirement for a high N.A. lens used in traditional optical trapping.Our system comprised a dual scanning system and two independent lasers which provided the ability to move the trapping spot independently of the confocal imaging process in close to real-time and without pre-programming. To demonstrate the efficacy of this innovativemethod, trapping and manipulation of live T cells was simultaneously performed over a field of view exceeding 1 mm2 for extended periods without compromising cell viability.