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Comparison of presumptive blood test kits including Hexagon OBTI

Tobe, S.S. and Daeid, N.N. (2009) Comparison of presumptive blood test kits including Hexagon OBTI. Journal of Forensic Sciences, 54 (1). p. 239. ISSN 0022-1198

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Abstract

Hochmeister et al. (1) performed validation tests for the Hexagon OBTI kit in 1999 and found a sensitivity of 1:100,000 when diluted in sterile water, a magnitude more sensitive than found in the current study under standard conditions. Hochmeister et al. (1) also conducted specificity, degradation, and contamination studies and determined that there was no effect on DNA recovery. This study was complimented by the work of Hermon et al. (2) who determined that sensitivity could be increased with a reduction of the buffer solution from 2 mL to 200 lL and also with an increase in incubation time. The maximum sensitivity that Hermon et al. (2) achieved was 1:1,000, even with an increase in incubation times, which they attribute to different volumes of blood used for the initial sampling. They also tested the specificity of the assay using different species of animals as well as other human body fluids.

Item type: Article
ID code: 19354
Keywords: Hexagon OBTI, blood test kits, Forensic Medicine. Medical jurisprudence. Legal medicine , Chemistry, Genetics, Pathology and Forensic Medicine
Subjects: Medicine > Public aspects of medicine > Forensic Medicine. Medical jurisprudence. Legal medicine
Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 02 Jun 2010 11:18
    Last modified: 05 Sep 2014 03:09
    URI: http://strathprints.strath.ac.uk/id/eprint/19354

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