Apperson, Kathleen and Karolin, Jan and Martin, Robert W. and Birch, David J.S. (2009) Nanoparticle metrology standards based on the time-resolved fluorescence anisotropy of silica colloids. Measurement Science and Technology, 20 (2). ISSN 0957-0233Full text not available in this repository. (Request a copy from the Strathclyde author)
We demonstrate nanoparticle size measurement using time-resolved fluorescence anisotropy decay in relation to establishing a nanometrology standard. The rotational correlation time equivalent to the isotropic Brownian rotation of a fluorescent 6-methoxyquinolinium dye attached to amorphous silica nanoparticles was determined in three different LUDOX* colloids from the complex fluorescence anisotropy decay observed. Once competing depolarization and nanoparticle aggregation had been taken into account, good agreement was found of 4.0 ± 0.4 nm, 6.4 ± 0.5 nm and 11.0 ± 1.6 nm corresponding to the manufacturer's reported particle radii of 3.5 nm, 6 nm and 11 nm, for LUDOX SM30, AM30 and AS40 respectively. We describe the measurement science required for acquisition and interpretation of fluorescence anisotropy decay data in order to determine nanoparticle size while highlighting the limitations and useful range of measurement.
|Keywords:||fluorescence decay time, fluorescence anisotropy decay, nanoparticle, nanometrology, colloidal silica, LUDOX, sol-gel, Solid state physics. Nanoscience, Instrumentation, Applied Mathematics|
|Subjects:||Science > Physics > Solid state physics. Nanoscience|
|Department:||Faculty of Science > Physics|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||01 Jun 2010 11:58|
|Last modified:||04 May 2016 15:52|