Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

The dose effect of human bone marrow-derived mesenchymal stem cells on epidermal development in organotypic co-culture

Laco, F. and Kun, M. and Weber, H.J. and Ramakrishna, S. and Chan, C.K. (2009) The dose effect of human bone marrow-derived mesenchymal stem cells on epidermal development in organotypic co-culture. Journal of Dermatological Science, 55 (3). pp. 150-160. ISSN 1873-569X

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

BACKGROUND: A wealth of evidences have shown the participation and benefits of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing and skin tissue repair in vivo. However, their role in epidermal development and reconstitution is not clearly investigated. OBJECTIVE: Here we examine the quantitative effect of human BM-MSCs on epidermal regeneration in vitro. METHOD: Human keratinocytes and BM-MSCs are cultured at ratios from 0% to 100% on top of a fibroblast-embedded collagen gel in a three-dimensional organotypic co-culture model at an air-liquid interface up to 20 days and analyzed by histochemical and immunochemical staining of filaggrin, involucrin and keratin 10 on days 14 and 20. Human BM-MSCs were tracked with quantum dots in organotypic co-cultures. RESULTS: It was found that epidermal development is strongly influenced by the percentage of co-cultured BM-MSCs. A strong chemotactic effect between keratinocytes and MSCs was seen in the group with 50% of BM-MSCs, which resulted in an impaired epidermal development, whereas at a low percentage of BM-MSCs (10%), a stratified epidermal structure resembling native skin was established on day 14 of culture. Moreover, the immunostaining studies revealed that BM-MSCs in the low percentage (10%) participated in the basal periphery of reconstructed epidermis and a similar pattern characteristic of native epidermis was demonstrated in this experimental group, which was superior to all other experimental groups in terms of the thickness of stratum corneum and the expression profile of epidermal differentiation markers. CONCLUSION: This study indicates the advantage of using a new skin equivalent model incorporating a small fraction of MSCs to develop biologically useful tissues for maintaining homeostasis during skin regeneration and wound healing process.

Item type: Article
ID code: 19018
Keywords: mesenchymal stem cells, organotypic, epidermis, skin regeneration, Medicine (General)
Subjects: Medicine > Medicine (General)
Department: Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 24 May 2010 11:34
    Last modified: 04 Oct 2012 13:13
    URI: http://strathprints.strath.ac.uk/id/eprint/19018

    Actions (login required)

    View Item