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Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage

Biggs, M.J.P and Richards, R.G. and Gadegaard, N. and McMurray, R.J. and Affrossman, S. and Wilkinson, C.D.W. and Oreffo, R.O.C. and Dalby, M.J. (2009) Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A (1). pp. 195-208. ISSN 1549-3296

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Abstract

Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotedulology has allowed the development of nanoscale substrates for the investigation into cell-nanofeature interactions. In this study human osteoblasts (HOBS) were cultured on ordered nanoscale pits and random nano "craters" and "islands". Adhesion subtypes were quantified by immunofluorescent microscopy and cell-substrate interactions investigated via immuno-scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO-1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nanoisland topographies; nanopits, however, were shown to inhibit directly the formation of large adhesions. STRO-1+ progenitor cells cultured on experimental substrates revealed significant changes in genetic expression. This study implicates nanotopographical modification as a significant modulator of osteoblast adhesion and cellular function in mesenchymal populations.

Item type: Article
ID code: 18980
Keywords: osteoblasts, mesenchymal stem cells, focal adhesions, genomic regulation, nanotopography, nanobioscience, scanning electron-microscopy, poly-l/dl-lactide, focal adhesion, osteoblast adhesion, matrix adhesions, dynamics, bone, mechanotransduction, differentiation, kinase, Electrical engineering. Electronics Nuclear engineering, Chemistry, Biology, Medicine(all)
Subjects: Technology > Electrical engineering. Electronics Nuclear engineering
Science > Chemistry
Science > Natural history > Biology
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 09 Jun 2010 16:21
    Last modified: 05 Sep 2014 03:58
    URI: http://strathprints.strath.ac.uk/id/eprint/18980

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