Picture of a sphere with binary code

Making Strathclyde research discoverable to the world...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. It exposes Strathclyde's world leading Open Access research to many of the world's leading resource discovery tools, and from there onto the screens of researchers around the world.

Explore Strathclyde Open Access research content

Establishing the mitochondrial DNA D-loop structure of Columba livia

Tsai, L.C. and Lee, J.C.I. and Liao, S.P. and Weng, L.H. and Linacre, A.M.T. and Hsieh, H.M. (2009) Establishing the mitochondrial DNA D-loop structure of Columba livia. Electrophoresis, 30 (17). pp. 3058-3062. ISSN 0173-0835

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The complete mitochondrial DNA D-loop structure of pigeon (Columba livia) was established in this study. A strategy of amplifying three partial fragments of the D-loop and then combing the three fragments to cover the full length of the D-loop was adopted. Ten samples from pigeons were collected and were successfully amplified and sequenced. Repetitive sequences of a VNTR and an STR were both observed at the 3-end of D-loop region. DNA sequence data revealed polymorphic sequences including indels, SNP, VNTR and STR within the D-loop. The size of the D-loop ranged from 1310 to 1327 bp from the initiation site of D-loop to the site immediately upstream of the repeat sequences depending upon the number of insertions or deletions. Each sample could be distinguished based on four genotyping procedures; being indels, SNPs, VNTRs and STRs. The polymorphic nature of the D-loop can be a valuable method for maternal identification and genetic linkage of pigeon in particular forensic science investigations.