Beltran, A. and Fontanals, N. and Marce, R.M. and Cormack, P.A.G. and Borrull, F. (2009) Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. Journal of Separation Science, 32 (19). pp. 3319-3326. ISSN 1615-9306Full text not available in this repository. (Request a copy from the Strathclyde author)
In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a -lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water. The molecularly imprinted polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a -lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 g/L with CFX were percolated through the tandem system.
|Keywords:||amoxicillin, cephalexin, molecularly imprinted polymer, river water samples, urine samples, Physical and theoretical chemistry, Filtration and Separation, Analytical Chemistry|
|Subjects:||Science > Chemistry > Physical and theoretical chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||21 Jun 2010 08:52|
|Last modified:||04 May 2016 16:25|