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Neuromuscular effects of four phospholipases A2 from the venom of Pseudechis australis, the Australian king brown snake

Geh, S.L and Rowan, E.G. and Harvey, Alan L. (1992) Neuromuscular effects of four phospholipases A2 from the venom of Pseudechis australis, the Australian king brown snake. Toxicon, 30 (9). pp. 1051-1057. ISSN 0041-0101

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Abstract

Four homologous single chain phospholipases A2 (Pa-1G, Pa-5, Pa-12C and Pa-15) were tested for neuromuscular effects on chick biventer cervicis and mouse hemidiaphragm nerve-muscle preparations. The four isozymes blocked directly elicited (mouse hemidiaphragm) and indirectly elicited (mouse and chick nerve-muscle preparations) twitch responses in concentrations of 1-30 micrograms/ml. The order of potency seen in both types of preparations was Pa-1G = Pa-5 greater than Pa-12C much greater than Pa-15. All four isozymes caused slow-onset, sustained contractures and reduction of muscle membrane potentials. In the chick preparation, responses to acetylcholine, carbachol and KCl were reduced by exposure to the toxins. It is concluded that the toxins act primarily postsynaptically to depress muscle contractility, perhaps by directly damaging muscle fibres. The order of potency agrees with their phospholipase A2 activity. Pa-1G is unusual because it is an acidic molecule, most toxic phospholipases being basic.

Item type: Article
ID code: 18523
Keywords: amino acid sequences, elapid snake, venom, toxicology, Pharmacy and materia medica, Toxicology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Dr EG Rowan
    Date Deposited: 17 Nov 2010 13:37
    Last modified: 05 Sep 2014 00:41
    URI: http://strathprints.strath.ac.uk/id/eprint/18523

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