Geh, S.L and Rowan, E.G. and Harvey, Alan L. (1992) Neuromuscular effects of four phospholipases A2 from the venom of Pseudechis australis, the Australian king brown snake. Toxicon, 30 (9). pp. 1051-1057. ISSN 0041-0101Full text not available in this repository. (Request a copy from the Strathclyde author)
Four homologous single chain phospholipases A2 (Pa-1G, Pa-5, Pa-12C and Pa-15) were tested for neuromuscular effects on chick biventer cervicis and mouse hemidiaphragm nerve-muscle preparations. The four isozymes blocked directly elicited (mouse hemidiaphragm) and indirectly elicited (mouse and chick nerve-muscle preparations) twitch responses in concentrations of 1-30 micrograms/ml. The order of potency seen in both types of preparations was Pa-1G = Pa-5 greater than Pa-12C much greater than Pa-15. All four isozymes caused slow-onset, sustained contractures and reduction of muscle membrane potentials. In the chick preparation, responses to acetylcholine, carbachol and KCl were reduced by exposure to the toxins. It is concluded that the toxins act primarily postsynaptically to depress muscle contractility, perhaps by directly damaging muscle fibres. The order of potency agrees with their phospholipase A2 activity. Pa-1G is unusual because it is an acidic molecule, most toxic phospholipases being basic.
|Keywords:||amino acid sequences, elapid snake, venom, toxicology, Pharmacy and materia medica, Toxicology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Dr EG Rowan|
|Date Deposited:||17 Nov 2010 13:37|
|Last modified:||06 Jan 2017 07:19|