Picture of a black hole

Strathclyde Open Access research that creates ripples...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of research papers by University of Strathclyde researchers, including by Strathclyde physicists involved in observing gravitational waves and black hole mergers as part of the Laser Interferometer Gravitational-Wave Observatory (LIGO) - but also other internationally significant research from the Department of Physics. Discover why Strathclyde's physics research is making ripples...

Strathprints also exposes world leading research from the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes

Sinclair, J.A. and Henderson, C.J. and Martin, I.K. and Grant, M.H. and Tettey, J.N.A. (2008) Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes. Journal of Pharmacy and Pharmacology, 60 (59). A24-A25. ISSN 0022-3573

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Aims to investigate the effect of liver digestion enzymes on the formation of potentially toxic reactive intermediates in suspensions of isolated rat hepatocytes. Isolated hepatocytes are recognized as one of the most relevant and practical models in drug metabolism and toxicity studies. Several modifications of the original twostage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there is little information on the effects of the liver digestion enzyme on glutathione (L-g-glutamyl-L-cysteinyl-glycine; GSH) conjugation of potentially reactive intermediates in isolated rat hepatocytes. Results indicate that the presence of trypsin inhibitor in the of isolated rat hepatocytes may increase the formation of GSH conjugates of potentially toxic reactive intermediates. This could have significant implications for the interpretation of metabolism data derived from hepatocytes in suspension.