Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes

Sinclair, J.A. and Henderson, C.J. and Martin, I.K. and Grant, M.H. and Tettey, J.N.A. (2008) Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes. Journal of Pharmacy and Pharmacology, 60 (59). A24-A25. ISSN 0022-3573

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Aims to investigate the effect of liver digestion enzymes on the formation of potentially toxic reactive intermediates in suspensions of isolated rat hepatocytes. Isolated hepatocytes are recognized as one of the most relevant and practical models in drug metabolism and toxicity studies. Several modifications of the original twostage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there is little information on the effects of the liver digestion enzyme on glutathione (L-g-glutamyl-L-cysteinyl-glycine; GSH) conjugation of potentially reactive intermediates in isolated rat hepatocytes. Results indicate that the presence of trypsin inhibitor in the of isolated rat hepatocytes may increase the formation of GSH conjugates of potentially toxic reactive intermediates. This could have significant implications for the interpretation of metabolism data derived from hepatocytes in suspension.

Item type: Article
ID code: 18406
Notes: Special Issue: BPC Science Abstracts. Poster Session 1: Drug metabolism
Keywords: trypsin inhibitor, gsh conjugates, toxic reactive intermediates, rat hepatocytes, bioengineering, Bioengineering, Engineering (General). Civil engineering (General), Pharmaceutical Science, Pharmacology
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Technology > Engineering (General). Civil engineering (General)
Department: Faculty of Engineering > Bioengineering
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Ms Ashley Urie
    Date Deposited: 26 Mar 2010 15:02
    Last modified: 05 Sep 2014 02:00
    URI: http://strathprints.strath.ac.uk/id/eprint/18406

    Actions (login required)

    View Item