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Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes

Sinclair, J.A. and Henderson, C.J. and Martin, I.K. and Grant, M.H. and Tettey, J.N.A. (2008) Trypsin inhibitor may increase the formation of GSH conjugates of potentially toxic reactive intermediates in isolated rat hepatocytes. Journal of Pharmacy and Pharmacology, 60 (59). A24-A25. ISSN 0022-3573

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Abstract

Aims to investigate the effect of liver digestion enzymes on the formation of potentially toxic reactive intermediates in suspensions of isolated rat hepatocytes. Isolated hepatocytes are recognized as one of the most relevant and practical models in drug metabolism and toxicity studies. Several modifications of the original twostage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there is little information on the effects of the liver digestion enzyme on glutathione (L-g-glutamyl-L-cysteinyl-glycine; GSH) conjugation of potentially reactive intermediates in isolated rat hepatocytes. Results indicate that the presence of trypsin inhibitor in the of isolated rat hepatocytes may increase the formation of GSH conjugates of potentially toxic reactive intermediates. This could have significant implications for the interpretation of metabolism data derived from hepatocytes in suspension.