Jiang, H-R and Al Rasebi, Z. and Mensah-Brown, E. and Shahin, A. and Xu, D. and Goodyear, C.S. and Fukuda, S.Y. and Liu, F.T. and Liew, Foo-Yew and Lukic, Miodrag L (2009) Galectin-3 deficiency reduces the severity of experimental autoimmune encephalomyelitis. Journal of Immunology, 182 (2). pp. 1167-1173. ISSN 0022-1767Full text not available in this repository. (Request a copy from the Strathclyde author)
Galectin-3 (Gal-3) is a member of the beta-galactoside-binding lectin family and plays an important role in inflammation. However, the precise role of Gal-3 in autoimmune diseases remains obscure. We have investigated the functional role of Gal-3 in experimental autoimmune encephalomyelitis (EAE) following immunization with myelin oligodendrocyte glycoprotein (MOG)35-55 peptide. Gal-3 deficient (Gal-3-/-) mice developed significantly milder EAE and markedly reduced leukocyte infiltration in the CNS compared with similarly treated wild-type (WT) mice. Gal-3-/- mice also contained fewer monocytes and macrophages but more apoptotic cells in the CNS than did WT mice. Following Ag stimulation in vitro, lymph node cells from the immunized Gal-3-/- mice produced less IL-17 and IFN-gamma than did those of the WT mice. In contrast, Gal-3-/- mice produced more serum IL-10, IL-5, and IL-13 and contained higher frequency of Foxp3+ regulatory T cells in the CNS than did the WT mice. Furthermore, bone marrow-derived dendritic cells from Gal-3-/- mice produced more IL-10 in response to LPS or bacterial lipoprotein than did WT marrow-derived dendritic cells. Moreover, Gal-3-/- dendritic cells induced Ag-specific T cells to produce more IL-10, IL-5, and IL-12, but less IL-17, than did WT dendritic cells. Taken together, our data demonstrate that Gal-3 plays an important disease-exacerbating role in EAE through its multifunctional roles in preventing cell apoptosis and increasing IL-17 and IFN-gamma synthesis, but decreasing IL-10 production.
|Keywords:||animals, apoptosis, cells, cultured, central nervous system, down-regulation/immunology, encephalomyelitis, autoimmune, galectin 3, glycoproteins, growth inhibitors, interleukin-10, male, mice, peptide fragments, Immunology, Medicine(all)|
|Subjects:||Science > Microbiology > Immunology|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||22 Mar 2010 17:00|
|Last modified:||01 Jul 2016 03:02|