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Modification of ionic currents underlying action-potentials in mouse nerve-terminals by the thiol-oxidizing agent diamide

Braga, M.F.M. and Rowan, E.G. and Harvey, A.L. (1995) Modification of ionic currents underlying action-potentials in mouse nerve-terminals by the thiol-oxidizing agent diamide. Neuropharmacology, 34 (11). pp. 1529-1533. ISSN 0028-3908

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Abstract

The effect of diamide, a thiol-oxidizing agent, was tested using electrophysiological techniques to determine whether its ability to alter neuromuscular transmission in vitro could be attributed to alterations of ion channels controlling neuronal excitability and/or acetylcholine release. In mouse triangularis sterni preparations, diamide transiently increased the evoked release of acetylcholine and then blocked release. Extracellular recording of perineural waveforms associated with neuronal action potentials at motor nerve terminals showed that diamide reduced the waveforms associated with the delayed rectifier K+ current, a Ca2+ current and a Ca2+-activated K+ current (ik,ca). Inhibition of quantal transmitter release was not associated with failure of action potentials to invade nerve terminals. Thus, diamide modifies the ionic currents underlying the nerve terminal action potential, some of these changes probably account for the complex effects of diamide on quantal transmission.

Item type: Article
ID code: 18358
Keywords: diamide, potassium channels, calcium channels, acetylcholine release, perineural waveform, neuromuscular transmission, Pharmacy and materia medica, Cellular and Molecular Neuroscience, Pharmacology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 07 Apr 2010 16:50
    Last modified: 05 Sep 2014 00:50
    URI: http://strathprints.strath.ac.uk/id/eprint/18358

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