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Determining biomolecular structures by time-resolved fluorescence

Rolinski, O.J. and Hernandez-Santana, A. and Graham, D. (2006) Determining biomolecular structures by time-resolved fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 6047 (60472N).

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Abstract

This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.