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Determining biomolecular structures by time-resolved fluorescence

Rolinski, O.J. and Hernandez-Santana, A. and Graham, D. (2006) Determining biomolecular structures by time-resolved fluorescence. Proceedings of SPIE - The International Society for Optical Engineering, 6047 (60472N).

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This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

Item type: Article
ID code: 18173
Notes: Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subseries of Proceedings of SPIE - International Society for Optical Engineering. Vol. 6047, Part 2. Progress in Biomedical Optics and Imaging, Vol. 7(2) Subseries of Proceedings of SPIE.
Keywords: time-resolved fret, maximum entropy method, dsdna, perylene, Optics. Light, Condensed Matter Physics, Electronic, Optical and Magnetic Materials, Electrical and Electronic Engineering, Applied Mathematics, Computer Science Applications
Subjects: Science > Physics > Optics. Light
Department: Faculty of Science > Physics
Faculty of Science > Pure and Applied Chemistry
Depositing user: Strathprints Administrator
Date Deposited: 14 Apr 2010 14:53
Last modified: 10 Dec 2015 18:42
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