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Fluorescence lifetime sensor of copper ions in water

Birch, D.J.S. and Rolinski, O.J. and Hatrick, D. (1996) Fluorescence lifetime sensor of copper ions in water. Review of Scientific Instruments, 67 (8). pp. 2732-2737. ISSN 0034-6748

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Abstract

We demonstrate an optical method for the selective detection of Cu(II) ions in water using time‐resolved fluorescence resonance energy transfer from the dye rhodamine 800 encapsulated in a sensor. In comparison to copper, quenching by other metal ions such as cobalt, nickel, and chromium is shown to be negligible. The experimental arrangement incorporates picosecond diode laser excitation and time‐correlated single‐photon counting for detection. Down to 5 mM of copper deposited on the sensor can be measured and a linear response is obtained up to at least 50 mM. A lower limit of detection for the sensor in the region of 10 ppb is shown to be readily achievable with good resolution.