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Open Access research that challenges the mind...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Fluorescence resonance energy transfer from allophycocyanin to malachite green

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (1999) Fluorescence resonance energy transfer from allophycocyanin to malachite green. Chemical Physics Letters, 309 (5-6). pp. 395-401. ISSN 0009-2614

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Abstract

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.