Picture of athlete cycling

Open Access research with a real impact on health...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the Physical Activity for Health Group based within the School of Psychological Sciences & Health. Research here seeks to better understand how and why physical activity improves health, gain a better understanding of the amount, intensity, and type of physical activity needed for health benefits, and evaluate the effect of interventions to promote physical activity.

Explore open research content by Physical Activity for Health...

A time-resolved near-infrared fluorescence assay for glucose: opportunities for trans-dermal sensing

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A time-resolved near-infrared fluorescence assay for glucose: opportunities for trans-dermal sensing. Journal of Photochemistry and Photobiology B: Biology, 54 (1). pp. 26-34. ISSN 1011-1344

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

We report a time-resolved near-infrared fluorescence assay for glucose detection that incorporates pulsed diode laser excitation. Reduction in fluorescence resonance energy transfer to a malachite green-Dextran complex from allophycocyanin bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The fluorescence quenching kinetics are analysed and discussed in detail. The change in fluorescence decay kinetics in the presence of glucose is found from dimensionality studies to be brought about by a change in the distribution of malachite green-Dextran acceptors. Glucose concentrations are measured in solution to within ±10% over the range 0-30 mM.