Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A method of determining donor-acceptor distribution functions in forster resonance energy transfer. Chemical Physics Letters, 324 (1-3). pp. 95-100. ISSN 0009-2614Full text not available in this repository. (Request a copy from the Strathclyde author)
We describe a method of determining the distribution function associated with acceptors partaking in Förster resonance energy transfer (FRET) from excited donors. Unlike previous approaches to this problem the method described makes no a priori assumption about the nature of the distribution. The use of an acceptor distribution function in analyte sensing is demonstrated for the first time. In the case of glucose sensing via FRET from Concanavalin A labelled with allophycocyanin to malachite green labelled dextran, the change in the acceptor distribution is shown to be a more sensitive sensing parameter than either the mean fluorescence lifetime or Förster γ value.
|Keywords:||forster resonance energy transfer, donor-acceptor, FRET, Physics, Physics and Astronomy(all), Physical and Theoretical Chemistry|
|Subjects:||Science > Physics|
|Department:||Faculty of Science > Physics|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||04 May 2010 15:42|
|Last modified:||04 May 2016 14:17|