A method of determining donor-acceptor distribution functions in forster resonance energy transfer

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A method of determining donor-acceptor distribution functions in forster resonance energy transfer. Chemical Physics Letters, 324 (1-3). pp. 95-100. ISSN 0009-2614 (http://dx.doi.org/10.1016/S0009-2614(00)00577-7)

Abstract

We describe a method of determining the distribution function associated with acceptors partaking in Förster resonance energy transfer (FRET) from excited donors. Unlike previous approaches to this problem the method described makes no a priori assumption about the nature of the distribution. The use of an acceptor distribution function in analyte sensing is demonstrated for the first time. In the case of glucose sensing via FRET from Concanavalin A labelled with allophycocyanin to malachite green labelled dextran, the change in the acceptor distribution is shown to be a more sensitive sensing parameter than either the mean fluorescence lifetime or Förster γ value.

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• Item metadata

  Item type:	Article
  ID code:	17484
  Dates:	Date Event 30 June 2000 Published
  Subjects:	Science > Physics
  Department:	Faculty of Science > Physics
  Depositing user:	Strathprints Administrator
  Date deposited:	04 May 2010 15:42
  Last modified:	08 Apr 2024 17:25
  URI:	https://strathprints.strath.ac.uk/id/eprint/17484