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A method of determining donor-acceptor distribution functions in forster resonance energy transfer

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A method of determining donor-acceptor distribution functions in forster resonance energy transfer. Chemical Physics Letters, 324 (1-3). pp. 95-100. ISSN 0009-2614

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Abstract

We describe a method of determining the distribution function associated with acceptors partaking in Förster resonance energy transfer (FRET) from excited donors. Unlike previous approaches to this problem the method described makes no a priori assumption about the nature of the distribution. The use of an acceptor distribution function in analyte sensing is demonstrated for the first time. In the case of glucose sensing via FRET from Concanavalin A labelled with allophycocyanin to malachite green labelled dextran, the change in the acceptor distribution is shown to be a more sensitive sensing parameter than either the mean fluorescence lifetime or Förster γ value.

Item type: Article
ID code: 17484
Keywords: forster resonance energy transfer, donor-acceptor, FRET, Physics, Physics and Astronomy(all), Physical and Theoretical Chemistry
Subjects: Science > Physics
Department: Faculty of Science > Physics
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 04 May 2010 16:42
    Last modified: 05 Sep 2014 01:11
    URI: http://strathprints.strath.ac.uk/id/eprint/17484

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