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World class computing and information science research at Strathclyde...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including by researchers from the Department of Computer & Information Sciences involved in mathematically structured programming, similarity and metric search, computer security, software systems, combinatronics and digital health.

The Department also includes the iSchool Research Group, which performs leading research into socio-technical phenomena and topics such as information retrieval and information seeking behaviour.

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A method of determining donor-acceptor distribution functions in forster resonance energy transfer

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2000) A method of determining donor-acceptor distribution functions in forster resonance energy transfer. Chemical Physics Letters, 324 (1-3). pp. 95-100. ISSN 0009-2614

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Abstract

We describe a method of determining the distribution function associated with acceptors partaking in Förster resonance energy transfer (FRET) from excited donors. Unlike previous approaches to this problem the method described makes no a priori assumption about the nature of the distribution. The use of an acceptor distribution function in analyte sensing is demonstrated for the first time. In the case of glucose sensing via FRET from Concanavalin A labelled with allophycocyanin to malachite green labelled dextran, the change in the acceptor distribution is shown to be a more sensitive sensing parameter than either the mean fluorescence lifetime or Förster γ value.