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Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (2001) Sensing metabolites using donor-acceptor nanodistributions in fluorescence resonance energy transfer. Applied Physics Letters, 78 (18). pp. 2796-2798. ISSN 0003-6951

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Abstract

Before fluorescence sensing techniques can be applied to media as delicate and complicated as human tissue, an adequate interpretation of the measured observables is required, i.e., an inverse problem needs to be solved. Recently we have solved the inverse problem relating to the kinetics of fluorescence resonance energy transfer (FRET), which clears the way for the determination of the donor-acceptor distribution function in FRET assays. In this letter this approach to monitoring metabolic processes is highlighted and the application to glucose sensing demonstrated.

Item type: Article
ID code: 17449
Keywords: metabolites, donor-acceptor nano-distributions, fluorescence resonance, energy transfer, nanoscience, fluorescence resonance energy transfer, fret, Solid state physics. Nanoscience, Physics and Astronomy (miscellaneous)
Subjects: Science > Physics > Solid state physics. Nanoscience
Department: Faculty of Science > Physics
Depositing user: Strathprints Administrator
Date Deposited: 21 Apr 2010 15:07
Last modified: 21 May 2015 11:33
URI: http://strathprints.strath.ac.uk/id/eprint/17449

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