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The facilitatory actions of snake venom phospholipase a(2) neurotoxins at the neuromuscular junction are not mediated through voltage-gated k+ channels

Fathi, B. and Rowan, E.G. and Harvey, A.L. (2001) The facilitatory actions of snake venom phospholipase a(2) neurotoxins at the neuromuscular junction are not mediated through voltage-gated k+ channels. Toxicon, 39 (12). pp. 1871-1882. ISSN 0041-0101

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Abstract

Electrophysiological investigations have previously suggested that phospholipase A2 (PLA2) neurotoxins from snake venoms increase the release of acetylcholine (Ach) at the neuromuscular junction by blocking voltage-gated K+ channels in motor nerve terminals. We have tested some of the most potent presynaptically-acting neurotoxins from snake venoms, namely β-bungarotoxin (BuTx), taipoxin, notexin, crotoxin, ammodytoxin C and A (Amotx C & A), for effects on several types of cloned voltage-gated K+ channels (mKv1.1, rKv1.2, mKv1.3, hKv1.5 and mKv3.1) stably expressed in mammalian cell lines. By use of the whole-cell configuration of the patch clamp recording technique and concentrations of toxins greater than those required to affect acetylcholine release, these neurotoxins have been shown not to block any of these voltage-gated K+ channels. In addition, internal perfusion of the neurotoxins (100 μg/ml) into mouse B82 fibroblast cells that expressed rKv1.2 channels also did not substantially depress K+ currents. The results of this study suggest that the mechanism by which these neurotoxins increase the release of acetylcholine at the neuromuscular junction is not related to the direct blockage of voltage-activated Kv1.1, Kv1.2, Kv1.3, Kv1.5 and Kv3.1 K+ channels.

Item type: Article
ID code: 17428
Keywords: beta-bungarotoxin, taipoxin, notexin, crotoxin, ammodytoxin, phospholipase A(2), voltage-gated K+ channels, neuromuscular junction, acetylcholine release, Science (General), Pharmacy and materia medica, Toxicology
Subjects: Science > Science (General)
Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 20 Apr 2010 14:21
    Last modified: 05 Sep 2014 01:15
    URI: http://strathprints.strath.ac.uk/id/eprint/17428

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