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Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum

Stokes, R.J. and McBride, E. and Wilson, C.G. and Girkin, J.M. and Smith, W.E. and Graham, D. (2008) Surface-enhanced raman scattering spectroscopy as a sensitive and selective technique for the detection of folic acid in water and human serum. Applied Spectroscopy, 62 (4). pp. 371-376. ISSN 0003-7028

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Abstract

Surface-enhanced Raman scattering (SERS) is shown to give linear and sensitive concentration-dependent detection of folic acid using silver nanoparticles created via ethylene-diaminetetraacetic acid (EDTA) reduction. Optical detection by SERS overcomes the primary limitation of photodissociation encountered during the application of other shorter wavelength ultraviolet (UV)/near-UV techniques such as fluorescence based microscopy. The SERS approach in water-based samples was demonstrated and optimized using several longer wavelengths of excitation (514.5, 632.8, and 785 nm). Excitation in the green (514.5 nm) was found to achieve the best balance between photodissociation and SERS efficiency. Linear concentration dependence was observed in the range of 0.018 to 1 lM. The importance of folic acid in a clinical setting and the potential applications of this technique in a biological environment are highlighted. We demonstrate the potential to transfer this technique to real biological samples by the detection of folic acid in human serum samples by SERS. (Abstract from : http://www.opticsinfobase.org/as/abstract.cfm?uri=as-62-4-371)

Item type: Article
ID code: 17083
Notes: http://suprimo.lib.strath.ac.uk/primo_library/libweb/action/display.do?ct=display&doc=SUVOY579586&indx=1&frbg=&dum=true&vid=SUVU01&vl(54032236UI0)=lsr02&vl(96071691UI1)=all_items&srt=rank&indx=1&dstmp=1272468249142&tab=local&ct=search&scp.scps=scope%3A(SU)&vl(freeText0)=Applied%20Spectroscopy&fn=search&mode=Basic&dscnt=0
Keywords: Raman spectroscopy, surface-enhanced Raman scattering, SERS, folic acid, nanoparticles, Chemistry, Therapeutics. Pharmacology, Pharmacy and materia medica
Subjects: Science > Chemistry
Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Pure and Applied Chemistry
Faculty of Science > Institute of Photonics
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    Depositing user: Strathprints Administrator
    Date Deposited: 28 Apr 2010 17:06
    Last modified: 17 Jul 2013 00:29
    URI: http://strathprints.strath.ac.uk/id/eprint/17083

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