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Multiplexed detection of six labelled oligonucleotides using surface enhanced resonance Raman scattering (SERRS)

Faulds, K. and Jarvis, R. and Smith, W.E. and Graham, D. and Goodacre, R. (2008) Multiplexed detection of six labelled oligonucleotides using surface enhanced resonance Raman scattering (SERRS). Analyst, 133 (11). pp. 1505-1512. ISSN 0003-2654

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Abstract

The labelling of target biomolecules followed by detection using some form of optical spectroscopy has become common practice to aid in their detection. This approach has allowed the field of bioanalysis to dramatically expand; however, most methods suffer from the lack of the ability to discriminate between the components of a complex mixture. Currently, fluorescence spectroscopy is the method of choice but its ability to multiplex is greatly hampered by the broad overlapping spectra which are obtained. Surface enhanced resonance Raman scattering (SERRS) holds many advantages over fluorescence both in sensitivity and, more importantly here, in its ability to identify components in a mixture without separation due to the sharp fingerprint spectra obtained. Here the first multiplexed simultaneous detection of six different DNA sequences, corresponding to different strains of the Escherichia coli bacterium, each labelled with a different commercially available dye label (ROX, HEX, FAM, TET, Cy3, or TAMRA) is reported. This was achieved with the aid of multivariate analysis, also known as chemometrics, which can involve the application of a wide range of statistical and data analysis methods. In this study, both exploratory discriminant analysis and supervised learning, by partial least squares (PLS) regression, were used and the ability to discriminate whether a particular labelled oligonucleotide was present or absent in a mixture was achieved using PLS with very high sensitivity (0.98-1), specificity (0.98-1), accuracy (range 0.99-1), and precision (0.98-1). (Abstract copied from Swetswise web site: http://www.swetswise.com/eAccess/viewAbstract.do?articleID=38598265)

Item type: Article
ID code: 17028
Notes: http://suprimo.lib.strath.ac.uk/primo_library/libweb/action/display.do?ct=display&doc=SUSFX954925263181&indx=3&frbg=&dum=true&vid=SUVU01&vl(54032236UI0)=lsr02&vl(96071691UI1)=all_items&srt=rank&indx=1&dstmp=1270735547697&tab=local&ct=search&scp.scps=scope%3A(SU)&vl(freeText0)=Analyst&fn=search&mode=Basic&dscnt=0
Keywords: multiplexed detection, labelled oligonucleotides, surfaceenhanced resonance, Raman scattering (SERRS)†, Chemistry, Physical and theoretical chemistry, Biochemistry, Spectroscopy, Environmental Chemistry, Analytical Chemistry, Electrochemistry
Subjects: Science > Chemistry
Science > Chemistry > Physical and theoretical chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 08 Apr 2010 15:12
    Last modified: 28 Mar 2014 05:15
    URI: http://strathprints.strath.ac.uk/id/eprint/17028

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